Each request received by the laboratory is considered an agreement between the laboratory and the sender. The obligations of both parties to that agreement are detailed on this site. If an organisation external to the Newcastle upon Tyne NHS Foundation Trust, wishes to procure our services, to a value in excess of £10,000 per annum then a Service Level Agreement will be drawn up between the two organisations.
- To provide a Cervical Screening service:
- To provide a diagnostic service:
- Post Vasectomy Semen Analysis:
- See also – Test Directory
The NHS Cervical Screening Programme invites all women between the ages of 25-49 and 50-64 to have a cervical screening test every three years and five years respectively.
Cervical screening is a method of preventing cervical cancer by detecting and treating abnormal changes in the cervix of women who are healthy and asymptomatic.
Specimens are received from all GPs and hospitals within the Newcastle, North Tyneside and Northumberland areas, the RVI processes about 50,000 specimens every year. (In the event that there are problems with the ICE electronic ordering, the attached CYGO015 form can be used for cervical screening cytology requests).
The specimens are taken directly from the cervix with a plastic brush, which is immediately placed into a vial containing SurePath preservative.
The lab receives the specimens, where they are processed in batches of 48 using Liquid Based Cytology methods.
Processing involves enrichment (which removes excess blood and mucus) and homogenisation. A random sample of cells, which is representative of the cells from the woman’s cervix, is transferred to the slide, which is then stained.
The samples are then analysed microscopically by qualified cytoscreeners and biomedical scientist for any dyskaryotic (pre-cancerous) cells.
Any samples that contain possible abnormalities are referred to, and interpreted by a Pathologist.
Any low grade abnormalities are triaged with a PCR test for High Risk HPV
Patients who do have abnormal samples will be monitored and/or treated accordingly.
Patients who have received treatment for dyskaryosis will have a Test Of Cure for HR HPV.
The cytology laboratory also receives samples from symptomatic patients who are suspected to have cancer, or to rule out malignancy.
Specimens are usually fresh fluids that can be collected relatively rapidly, painlessly and simply, these include urines, bladder washes, CSF, sputum, bronchial washes, serous fluids and fine needle aspirates.
Fresh fluids are received in universal containers, and are spun down to concentrate any cells in the fluid that are then transferred via cytocentrifugation to glass slides (fine needle aspirate samples can be spread directly onto slides).
Non-gynae specimens can also be processed using Liquid-Based Cytology methods.
The specimens are then stained with Papanicolou and May Grunwald Giemsa so that the Pathologists can interpret them microscopically. The Pathologists may then request further special stains and/or immunocytochemistry to help them reach a diagnosis.
This is a screening test only, checking for the absence of sperm after a vasectomy. It is not to be used for fertility assessment (number and vitality of spermatozoa). If this is required please refer the patient to Newcastle Fertility Centre for Life.
How to collect a semen sample for post-vasectomy sperm count:
Before you collect your sample:
- Samples should be collected after a minimum of two days but no longer than seven days of sexual abstinence.
To collect your sample:
- Use the container provided for your sample. Do not use a condom to collect your specimen as these are harmful to sperm.
- It is very important that the entire specimen goes into the container and none is lost. If some sample is lost, select ‘N’ for the Complete Sample? Question.
- Ensure that the container is securely closed.
Please label the specimen container with:
- Your full name and date of birth
- The type of specimen (semen)
- The date and time the specimen was produced
Please ensure that the request form provides the following information:
- Your full name, address and date of birth
- Your GP’s name and surgery OR referring clinician.
- The date of your vasectomy
- The date and time the specimen was produced
- NB: Incompletely labelled specimens or forms may not be processed
Delivering your specimen to the hospital:
- Keep the specimen at room temperature or as near to body temperature as possible (for example, by transporting it in your pocket)
- Your specimen must be delivered within 2 hours of production to the Department of Cytology at the Royal Victoria Infirmary (in the New Victoria Wing, Level 3) on any week day (Monday to Friday) 8:30am to 3:30pm. Please ensure the sample is handed to a member of staff.
Please note that this service is not accredited by UKAS to CPA or ISO 15189:2012